Neuron culture medium: 0.1 ×106
Conventional culture medium: 0.5～1.0×106
Number of cells: 0.1×106(isolated from the hippocampus of a mouse at embryonic days 18.5)
Number of cells: 0.1×106
Number of cells: 0.1×106
Number of cells: 0.1×106
Number of cells: 0.1×106
▶Ca2+
Seeding density: 500 μL/well(polylysine-coated glass bottom dish)
Ca2+
